PURPOSE: 1 To build high pressure cell culture model in vitro of Retinal Ganglion Cells(RGCs) and observe the RGCs effected by pressure .
目的：1 建立高压体外培养视网膜神经节细胞模型并观察压力对视网膜神经节细胞的影响；
METHODS Based on cell culture model in vitro, MTT test was used to study the inhibitory effect of curcumin derivatives on tumor cell lines.
方法利用体外细胞培养模型，应用MTT法分析姜黄素衍生物对不同肿瘤细胞生长的影响。
METHODS:Methods of in vitro cell culture model of human primary PDLC, tetrazolium bromide reduction (MTT) assay and direct contact were used.
方法：体外细胞培养，MTT （噻唑蓝）比色法，材料直接接触法。
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