...—拟南芥CBF1基因的克隆分析与表达载体构建 关键词 CBF1基因；转录因子；克隆；载体构建；拟南芥 [gap=678]Key words: CBF1 gene;transcriptional factor;Agribacterium tumefacien;transform;maize SAUMZ1..
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transcription factor CBF1 gene 转录因子CBF1基因
PCR assaying of plant DNA confirmed that the CBF1 gene has been integrated into the strawberry genome.
转化植株经pcr检测，证实了CBF1基因已经整合到草莓的基因组中。
CBF1 gene from rape was transformed into Arabidopsis thaliana by Agrobacterium tumefaciens-mediated method to screen its transgenic plants with resistance and conduct PCR detection and GUS staining.
采用根癌农杆菌介导法将油菜CBF1基因转入拟南芥，筛选抗性转基因拟南芥植株，进行PCR检测和GUS染色。
The degree of the effects depended on the expression level CBF1. Proper regulation strategy for gene expression should be concerned when using CBF1 to modified plants.
因此，利用CBF1基因进行植物遗传改良时应根据物种的应用特点采用合适的表达调控策略。
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