Methods: To refer to the method stipulated by USP with 0.50% CaCl2 solution instead of 0.25% CaCl2 solution.

方法：参照美国药典方法，将其中的0.25％氯化钙溶液用0.50％氯化钙溶液替代。

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Conclusion: 0.50% CaCl2 solution can substitute for 0.25% CaCl2 solution and has the superiority of reducing the volume of 0.50 clotting.

结论：采用0.50%氯化钙溶液可替代0.25%氯化钙溶液，且比现行方法具有降低羊血浆半凝固度剂量的优点。

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