Methods Taking inhibition of in vitro tumor cells as active indexes,a bioassay-guided separation and toxicity evaluation in mice of ingredients in Bufonis Venenum were applied.
方法采用追踪分离的方式,以体外肿瘤细胞生长抑制强度为活性指标,同时结合小鼠急性毒性实验,对蟾酥的主要化学成分进行初步研究。
参考来源 - 蟾酥抗肿瘤有效成分的活性追踪分离及急性毒性研究·2,447,543篇论文数据，部分数据来源于NoteExpress
Results Venenum Bufonis had growth inhibitory and apoptosis-inducing effects on U2OS cell with a time and dose dependent relation.
结果蟾酥水提取物对U2 OS细胞具有生长抑制及诱导凋亡作用，且呈时间依赖和剂量依赖。
Results: The optimum extraction technology of Venenum Bufonis is using 90% and 80% alcohol extraction.
结果：蟾酥最佳提取工艺为采用90 %、80 %乙醇依次提取。
Methods: Extracted Venenum Bufonis remains was determined by physical and chemical test and TLC.
方法：采用理化鉴别和薄层色谱鉴别法对提取后的蟾酥残渣进行检查。
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