Objective To examine the changes of conformation and activity of human placental alkaline phosphatase(HPAP) in denaturation and renaturation of guanidine chloride, and to study its folding mechanism.

目的观测人胎盘碱性磷酸酶（HPAP）在盐酸胍变性和复性过程中构象与活力的变化，探讨其折叠机制。

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Objective To study the effects of phosphate on guanidine denaturation of human placental alkaline phosphatase (HPAP) and provide a theoretical basis for flexibility hypothesis of enzyme active sites.

目的观察磷酸盐对人胎盘碱性磷酸酶(HPAP)胍变性的影响，为酶活性部位的柔性理论提供证据。

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