The expressed protein was purified by one-step affinity chromatography, and its ability of binding plasmid was investigated via gel retardation experiments.
表达产物用亲和层析一步法进行纯化，然后用凝胶阻滞试验观察重组蛋白结合质粒的能力。
Methods: MMA was prepared with asialo-fetuin affinity and gel-filtration chromatography form murine melanoma.
方法：利用亲合层析及凝胶过滤层析从小鼠黑色素瘤中提取小鼠黑色素瘤凝集素（MMA）。
The recombinant protein was purified through Ni-chelating affinity chromatography, and the purity was above 90% explained by SDS-PAGE gel scan analysis.
利用镍柱亲和层析对表达的重组蛋白进行纯化，经SDS-PAGE及薄层扫描分析表明，重组蛋白的纯度在90%以上；
应用推荐