限制位点（restriction site）：特定限制酶所具有的 特异识别的DNA序列 。 粘性末端（cohesive end）：DNA经限制酶切开后产生 的一种交错切口。
基于36个网页-相关网页
这种技术可在DNA靶标分子的任意位点进行基因敲除、敲入、点突变等操作，无需使用限制性内切酶和连接酶。
The Red mediated recombination can be used to insert, delete or substitute DNA sequences at any desired position on a target molecule without the need for restriction enzymes or DNA ligases.
然而其缺乏与细胞的识别位点以及自身的强疏水性使其应用受到限制。
However, its use is limited by lacking of cell recognition sites and their hydrophobic property.
在研究突变机制时，发现有6例由于核苷酸的替换引起了限制性位点的增减。
In the study of mutation mechanism, it was found in 6 cases that there were gains and losses of sites due to nucleotide substitution.
应用推荐