目的评价菌落pcr法鉴定噬菌体抗体库阳性重组率的可靠性。
Objective To evaluate the reliability of colony PCR in identifying the recombinant clones selected from phage antibody libraries.
结论：异种血管内皮生长因子基因重组T7噬菌体疫苗可打破机体对自身vegf的免疫耐受，诱导产生较高水平的特异性抗vegf抗体。
Conclusion: Recombinant T7 phage vaccine expressing xenogenic VEGF can break immunologic tolerance against self-VEGF and induce the producing of specific anti-VEGF antibody.
应用推荐