使用丙肝病毒重组免疫印迹分析技术和丙肝病毒核糖核酸检测对丙肝病毒感染进行确诊。
HCV recombinant immunoblot assay (RIBA) and HCV RNA testing are used to confirm the diagnosis of HCV infection.
间接免疫荧光、ELISA和免疫印迹检测证明,含SO . 7片段的三种重组杆状病毒均在昆虫细胞中表达出相应的活性蛋白。
By IFA, ELISA and Western blot experiments, it was proved all the recombinant baculoviruses containing SO. 7 segment could express biologically active proteins in insect cells.
结果表明:所获得的重组NP蛋白在ELISA分析中均能与阳性血清和单克隆抗体发生特异性结合,但在免疫印迹分析中仅与阳性血清发生特异性结合。
The results showed recombinant NP protein could bind to both positive serum and MAb with specificity in ELISA, but only bind to positive serum in western blot.
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