114 GluR2 含量测定 采用细胞比色分析( colorimetric assay) 法进行: ①膜表面 GluR2 含量测定: 常规固定,加鼠抗 GluR2 (Chemicon 公司) N2末 端一抗,4 ℃过夜;加 HRP 二抗(Amersham公司) ,...
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采用葡萄糖氧化酶法检测3T3-L1葡萄糖的消耗作用,使用油红O染色并通过比色定量分析3T3-L1细胞的脂肪含量。
The glucose consumption in 3T3-L1 cells was determined by glucose oxidase(GOD)method. The fat content in 3T3-L1 cells was determined by Oil Red O staining and spectrophotometry.
采用MTT比色法、平板克隆形成实验、流式细胞术和MTS比色法分析SLP鄄2对TE12细胞的影响。
MTT assay, plate clone formation assay, flow cytometry, and MTS assay were performed to measure the effect of SLP-2 on TE12 cells.
采用MTT比色分析法检测细胞增殖情况。
MTT colorimetry was used to determine the proliferation of SW1116 cell line.
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