目的：利用聚合酶链反应定点突变技术构建人血小板反应素1基因第13外显子编码钙结合域突变体。
AIM: To establish the mutant of coding calcium binding fragment of the 13th exon of human thrombospondin-1 (TSP-1) gene with polymerase chain reaction (PCR) site directed mutagenesis technology.
用马铃薯淀粉柱可以直接分离麦芽糖结合蛋白乳酸脱氢酶辅酶结合结构域融合蛋白，并得到满意的结果。
A method for the purification of expressed maltose binding protein fused protein by using potato starch instead of crosslinked amylose have been developed.
蛋白结构域分析表明，水稻泛素结合酶基因主要由一个泛素结合酶催化结构域组成。
Protein domain analysis showed that ubiquitin-conjugating enzyme genes mainly consist of a big ubiquitin-conjugating enzyme catalytic domain.
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