RNA的多聚酶反应（RT-PCR）是以mRNA 为 模板进行逆转录反应（reverse transcription，RT） 与PCR，可用于检测单个细胞或少数细胞中少于 10个拷贝的RNA模板。
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方法：采用逆转录巢式酶链免疫反应，对41例结直肠癌组织及其配对的癌旁正常组织和25例良性腺瘤的FHIT基因进行检测。
Methods: Matched normal and cancerous tissues from 41 patients with colorectal cancer and 25 patients with colorectal adenoma were examined for FHIT expression using RT-PCR.
方法应用逆转录酶-多聚酶链反应(RT - PCR)方法，检测了83例食管癌和贲门癌组织及28例患者45枚淋巴结中MRP基因的表达，并与相应癌旁组织进行对照分析。
Methods RT-PCR was applied to study expression of MRP gene in tumor tissues from 83 cases of esophageal and cardiac carcinoma, and 45 lymph nodes from 28 patients.
方法从Z 10病毒感染的细胞提取总rna，将逆转录聚合酶链反应(RT PCR)扩增的产物纯化后克隆于t载体并进行序列测定，应用dnas TAR软件分析比较。
Methods the total RNA were prepared from Z10 virus infected cells and the RT PCR products was cloned into t vector, sequenced and analyzed by using DNASTAR software.
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