通过观察细胞病变效应,用噻唑蓝(MTT)比色法检测细胞存活率,以确定该药物治疗指数。
By means of observing cytopathic effect and adopting MTT colorimetric assay for cell survival rate, their therapeutic index was calculated.
方法:通过观察药物毒性、病毒引起的细胞病变效应、MTT法检测细胞活性,判断药物毒性及药物抗病毒效应。
Methods: The toxic and antiviral effect was evaluated by observing toxic effect, cytopathic effect (CPE) and MTT colorimetric assay for vital cell rate.
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