...表达条件优化；同源模建分析 [gap=1468]Key words: molecular biology; cytochrome P450nor2; optimization of expression conditions; homology modeling ...
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关键词：原核表达；表达条件优化；重组蛋白；生物活性 [gap=1566]Key words：pmkaryotie expression；optimal expression condition；recombinant protein；biological activity
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目的：优化融合表达抑肽酶基因工程菌的发酵条件，分离纯化抑肽酶。
Aim: to optimize the fermentation conditions of engineering bacteria expressing fusion proteins composed of aprotinin and to purify aprotinin.
利用GUS基因的酶学特性，进行瞬时表达研究，目的是在短期内优化转化条件。
Utilizing enzymology trait of GUS gene, we did instantaneous expression experiments so that we could optimize the transgenic conditions in short run.
对大肠杆菌表达的重组人胰岛素原包涵体蛋白的变性复性条件进行了优化。
Optimization of the renaturation conditions for recombinant human proinsulin inclusion bodies expressed in E.
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