...骨机械法分离培养成骨细胞,成骨细胞利用碱性磷酸酶染色(Alkaline phosphatase staining,ALP)、茜素红染色(Alizarin Red staining)进行鉴定。
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成骨诱导后茜素红染色阳性，对照组为阴性。
Oil red staining and alizarin red staining both demonstrated positive reactions but not in control groups.
主要观察指标：形态观察、增值率测定、碱性磷酸酶染色、细胞基质钙含量测定、茜素红染色显示矿化结节数。
MAIN OUTCOME MEASURES: Morphological observation, determination of proliferation ratio, ALP stain, determination of matrix calcium accumulation and number of mineralized nodes with alizarin red stain.
茜素红s染色见钙结节形成。
Alizarin red s staining verified the formation of mineralized nodules.
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