制备了以壳聚糖为载体的金属螯合亲和色谱分离纯化还原型谷胱甘肽(GSH)。
Immobilized metal ion affinity chromatography sorbents were prepared for purification of reduced glutathione (GSH) using chitosan as substrate.
方法采用DEAE -纤维素柱色谱和凝胶渗透色谱分离纯化，化学和光谱方法分析其结构特征。
Methods DEAE-Cellulose and gel-filtration chromatography were used to isolate and purify the polysaccharides, whose structures were characterized by chemical and spectral methods.
该法简便、不需要中间产物的分离纯化、操作放射性步骤少、制备周期短，产品用高压液相制备色谱分离纯化，效率高、纯度在98%以上。
It reduces manipulations of large amounts of radioactivity. The product was isolated by preparation HPLC. Its radiochemical purity is higher than 98%.
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