通过外科手术对小鼠左右侧颈总动脉进行结扎建立小鼠脑缺血再灌注损伤模型(cerebral ischemia reperfusion injury,CIR/I),在术前30mins给小鼠尾静脉注射不同剂量的CAPE。
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方法用线栓法制作脑缺血再灌注损伤模型。
Methods Rat cerebral ischemia -reperfusion injury model was established with suture emboli method.
目的：观察不同剂量的纳洛酮对大鼠局灶性脑缺血再灌注损伤模型的影响，并探讨其保护作用机制。
OBJECTIVE: to study the influence of different doses of naloxone on focal cerebral ischemia reperfusion injury in rats and to explore its mechanism.
方法：采用可逆性脑缺血再灌注损伤大鼠模型，通过电针“人中”穴、双侧“中冲”穴及“风府”穴，利用CK及LDH测试盒测试酶活性。
Methods: adopt reversible cerebral ischemia reperfusion injury model in rats, acupuncture the points - "Renzhong", "Zhongchong" and "Fengfu", calculate the content of CK and LDH using testing box.
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