食用仙人掌的离体培养及其快繁技术 关键词：食用仙人掌；分化；继代增殖；生根 [gap=1353]Key Words:opuntia milpa alta haw, differentiation, mulitiplication, rooting
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The optimizing medium for subculture multiplication was MS+6-BA0.2mg/L+ IAA0.2mg/L + AC0.4g/L. After 30 days culture, the multiplication coefficient was 83.2%.6.
5、继代增殖的最适培养基为MS+6-BA0.2mg/L+IAA0.2mg/L+AC0.4g/L,30d后其平均增殖系数可达83.2%。
参考来源 - 柠条锦鸡儿组织培养关键技术的研究·2,447,543篇论文数据，部分数据来源于NoteExpress
使用单因子的生长素naa或IAA不利于百合的继代增殖。
Choosing one-factor auxin was adverse to buds subculture multiplication of Lily, for example NAA or IAA.
结果表明浅层液体培养继代增殖系数、生根长度及株高均优于固体培养；
The multiplication coefficient? the length of root and plant height in shallow liquid culture were prior to those of solid culture.
愈伤组织继代增殖分化出大量无根小苗，再诱导生根成苗，然后炼苗、田间移栽、成活率达87%。
Callus was formed and differentiated into numerous rootless plantlets, which were induced to rooting. After hardening off, the rooting plantlets were transplanted to nursery.
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