包涵体蛋白经含8m尿素的结合缓冲液溶解后，用金属亲和层析法进行纯化，获得了高纯度的目的蛋白。
After dissolution of inclusion bodies in binding buffer containing 8m Urea, the fusion protein was purified with metal affinity chromatography column, high purity protein was achieved.
多年来，人们常假定孵育后的细胞再用缓冲液洗涤时，能导致细胞膜结合多肽的解吸附作用。
Over the years, it has frequently been assumed that washing the cells with buffer after incubation causes desorption of peptide associated with the plasma membrane.
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