本研究中,我们首先采用酶联免疫吸附试验(ELISA)和间接免疫荧光(IFA)测定法对107单抗的结合特异性(binding specificity)进行检测,随后,我们利用已建立的简单高通量的微中和试验(ELISPOT-MNT)法对筛选出来的94株单抗的中和活性谱进行全面的检测和分析。
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目的和方法:用噬菌体随机7肽库对神经胶质瘤母细胞(SWO - 38)进行全细胞筛选,并通过竞争结合实验检验所筛选出的克隆的结合特异性。
AIM and METHODS: The Ph. D. -7 phage display library was used to isolate peptides specific for glioma SWO-38 cell by whole cell screening.
抗体具有非常特异性,每种抗体只识别和结合一种蛋白质区域。
Antibodies are very specific in that each antibody will recognize and bind to one protein region only.
研究者们接下来通过特异性结合细胞表面蛋白质的抗体将神经胶质瘤细胞分成不同的类型。
The researchers then sorted glioma cells into different types using antibodies that stick to specific proteins on a cell's surface.
So, I have a bacteria infection, stimulates my immune system, I start making antibodies that bind to an antigen specific to that bacteria.
所以 如果我被细菌感染了,我的免疫系统被激活,开始制造抗体,这些抗体结合到细菌特异性的抗原上
Those receptors, some fraction of them, are specific for the ligand that the pre-synaptic cell releases.
这些受体 其中一部分,特异性地结合突触前细胞释放的配体
From a technological perspective, antibodies are incredible tools because antibodies are molecules that are specifically designed to bind to a particular antigen or a particular chemical.
从技术角度上来说,抗体是一种令人难以置信的分子,它们被设计来,特异性地结合抗原,或者某种化学物质
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