利用计算机图像分析仪进行灰度扫描,灰度值越小,染色程度越高,即血管细胞黏附因子1的免疫沉积越多。
The smaller the gray value, the higher the staining degree was, and the more the immune deposition of VCAM 1 was.
常规石蜡切片,磷钨酸苏木精染色,图像分析仪测量心肌细胞的胞面积、核面积、核浆比和核椭圆度。
Routine paraffine sections were made for PTAH staining and data of cell area, nuclear area, ratio of nucleus to cytoplasm, nuclear ellipticity were measured by image computer analyzer.
方法根据不同时期树突状细胞的粘附性质不同,设计简便的树突状细胞的体外扩增和纯化方法,并利用瑞氏染色和流式细胞仪(FACS)鉴定其生物学特征。
Methods According to different adhesiveness of DC in vary period, a simple method was used to generate and purificate DC, and their biology character were identified by Wright staining and FACS.
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