结论抗体抗原亲和力随免疫次数的增加趋向饱和，BSA的线性表位为优势表位，随免疫次数的增加比重逐渐增强。

Conclusion With the increase of the immunization times, the affinity of antibody come to mature and the proportion of dominant linear epitopes of BSA became higher.

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为获取识别多种WSSV抗原的抗体提供了新的方法路线， 也为获取特异性识别线性表位的单链抗体提供了一种新的淘选技巧。

This study provides a new way to obtain more different antibodies specifically binding to WSSV, and especially provides a new strategy to obtain scFvs against linear epitopes.

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