...一、目的： 学习聚丙烯酰胺凝胶平板等电聚焦电泳测定蛋白质等电点的原理及方法。 二、原理： 等电点聚焦（isoelectric focusing, IEF）或简称电聚焦（electrofocusing），也曾称等电点分离聚焦电泳等。它是60年代中期出现的技术，克服了一般电泳易扩散的缺点。
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... IEF - Isoelectric focusing 等电点聚焦 Isoelectric Focusing system 双向电泳系统 isoelectric focusing IEF 等电聚焦 ...
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等电点聚焦电泳 [生化] isoelectric focusing electrophoresis ; Isoelectric Focusing ; IEF
凝胶等电点聚焦 GEF
等电点聚焦简称电聚焦 isoelectrofocusing ; IEF
等电位点聚焦 isoelectric focusing ; IEF
间蛋白质点在等电聚焦 isoelectric focusing ; IEF
在等电点聚焦分析中该成分为一条考马斯亮蓝染色带。
Isoelectric focusing of STFC in thin-layer polyacrylamide gel obtained only one band as shown by Coomassie Blue staining.
方法：采集33例正常人，26例牙周炎患者全唾液，运用聚丙烯酰胺凝胶等电聚焦方法观察其蛋白质等电点分布。
Methods: Saliva was collected from 33 healthy and 26 periodontitis individuals respectively, and analysed with isoelectric focusing in polyacrylamide gels.
蛋白质首先在毛细管等电聚焦过程中根据它们等电点的差异得到分离，然后被电喷雾质谱鉴定。
Proteins were separated and resolved in CIEF according to their differences in isoelectric point (pI), and then characterized by ESI MS.
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