构建无动物源性的人胚胎干细胞培养体系的研究 -- --万方医学网-医学杂志-医学期刊-医学论文-中华医学会期刊-中国医师协会期刊-医学外文文献 A-4,TRA-1-60和荧光定量PCR检测多能性因子Oct3/4，hTERT的表达，以及ELISA测定各种培养体系的条件培养基(conditioned medium，CM)中bFGF浓度的变化。同时对AF培养体系中长期传代后的hESCs进行核型及干细胞特性的相关检测。
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细胞的条件培养基 Kupffer cell conditioned medium
的内皮细胞条件培养基 endothelial cell conditioned media
的单核细胞条件培养基 monocyte conditioned media
用表达ntn的条件培养基处理原代培养胚胎中脑神经元，发现其有促进神经元存活和发育功能。
It was found that NTN can promote survival and growth of neurons when culturing primary embryonic mesencephalic neurons with conditioned medium contained NTN.
来自细胞悬浮培养物的条件培养基能显著地促进人参培养细胞的单细胞在较低细胞植板密度培养时的克隆形成。
Conditioned medium (CM) stemming from cell suspension culture could remarkably promote the formation of single cell clone from culture cells of Panax ginseng below a critical cell plating density.
目的探索从骨肉瘤细胞条件培养基中提取骨形态蛋白（BMP）的方法，并测定其生物学活性。
Objective: To find out a method of extraction and purification of bone morphogenetic protein (BMP) from osteosarcoma cell conditioned medium, and evaluate the biological activity of BMP.
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