由此进行单引物PCR扩增,它们的扩增产物形成了稳定的引物——模板特异的DNA指纹图谱。
The products amplified by single-primed PCR formed DNA fingerprints with the specificity of primer-template.
选择已经定位染色体的SSR引物对单染色体进行PCR扩增,根据得到的分子标记进行单染色体的鉴定。
Select the SSR primer whose colorant layer is located to do PCR augmentation to the monosome, and certificate the monosome according to the gained molecular label.
反向引物SA4或EM6与研究的所有正向引物组合时产生的多态性条带分子量完全相同, 这些条带可能是由反向单引物扩增而来的。
The reverse primers SA4 or EM6 produced identical polymorphic bands when combined with all the forward primers tested. These bands might be amplified by the reverse primers.
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