为今后的生物监测提供可靠的半致死浓度的数据。

The median lethal concentration data is reliable for future biological observation.

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结果表明，苜蓿愈伤组织经DES处理，DES的半致死浓度为0 3% ，愈伤组织的抗寒性增强。

The DES concentration resulting in 50% death was 0.3%, and the cold resistance of alfalfa callus was increased.

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