方法采用环介导恒温扩增反应（LAMP）技术扩增轮状病毒特异性基因，并与传统PCR技术检测结果比较。

Methods The specific gene of rotavirus was amplified by LAMP, and compared with the detective result by PCR.

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目的建立环介导的恒温扩增快速检测大肠杆菌O157的方法。

Objective To establish a method of Loop-mediated isothermal amplification(LAMP) for the detection of specific gene from Bacterium coli in clinic samples.

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