本发明公开了一种用于检测非洲猪瘟病毒抗体的竞争ELISA试剂盒及其用途,属于生物技术领域。
The invention discloses a competitive ELISA kit for detecting african swine fever virus antibody and the purposes thereof, which belong to the biological technical field.
试剂盒采用原核表达的重组P 30蛋白作为包被抗原,依据竞争ELISA原理检测猪血清中非洲猪瘟病毒的抗体。
The kit adopts prokaryotic expression recombinant P30 protein as coating antigen, and detects the African swine fever virus antibody in pig serum according to a competitive ELISA principle.
本研究成功制备了抗猪瘟病毒NS3蛋白单克隆抗体,为深入研究NS3蛋白在猪瘟病毒致细胞病变中的作用奠定了基础。
In the end, we produced anti-CSFV NS3 monoclonal antibodies successfully, which were foundation for the further study on function of NS3 protein in CPE caused by CSFV.
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