方法采用多对型特异性引物-聚合酶链反应检测160例慢性乙型肝炎患者血清HBV基因型;
Methods: Serum samples from 160 cases with chronic HBV infection were collected and tested for HBV genotypes by type-specific primers.
引物的特异性和灵敏度采用基因型已知的质控DNA进行验证。
Control DNA samples that genotypes known were used to confirm the sensitivity and specificity of each sequence-specific primer.
结论采用寡核苷酸阵列芯片技术可对HCV进行基因型以及基因亚型的分析,方法快速、简便、敏感、特异。
Conclusions This method is highly specific, sensitive and rapid for the diagnosis of the genotypes and sub-genotypes of HCV.
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