方法：细胞毒测定以MTT法； 单药细胞毒测定以半倍浓度梯度设计；
Methods: Cytotoxicity of manumycin was determined by MTT assay, and cytotoxicity of the other drugs alone was determined using the half concentration gradient design.
浓度梯度按等对数间距设置，半致死浓度采用直线内插法进行计算。
The grades were designed by equal space between logarithm, and the median lethal concentration was computed by Linear Regression.
首次提出了针对高浓度有机质的活性污泥间歇浓度梯度驯化法，阐明了驯化过程中糖代谢速率和产氢能力的演变规律。
This is the first to our knowledge reported for the batch acclimation of sludge at high concentration of organic matter medium over 80g sucrose per liter.
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