...文 vivin，并转染DU-145细胞，RT-PCR检测细胞中survivin基因的mRNA表达；MTT法和流式细胞仪(Fluorescein Activated Cell Sorter，FACS)分别检测genistein与RNA干扰survivin联合使用对细胞增殖和细胞周期的影响。
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...研究 vivin,并转染DU-145细胞,RT-PCR检测细胞中survivin基因的mRNA表达;MTr法和流式细胞仪(FluoresceinActivatedCellSorter,FACS)分别检测genistein与RNA干扰survivin联合使用对细胞增殖和细胞周期的影响.
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方法采用MTT法和流式细胞仪技术。
应用MTT法和流式细胞仪检测系膜细胞增殖、凋亡和细胞周期。
Proliferation, apoptosis, and cell cycle of HMCs were examined by MTT assay and flow cytometry respectively.
结果形态学变化、流式细胞仪分析和TUNEL法结果均证实含鲜壁虎血清在体外可诱导C6胶质瘤细胞凋亡；
Results The C6 glioma cell apoptosis induction of GFP contained serum in vitro could be confirmed by morphological examination, flow cytometry analysis and TUNEL method.
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