结果构建的EBO G87和EBO WT重组载体经内切酶双酶切鉴定及核苷酸序列测定证实。
Results The constructed vectors of EBO-WT and EBO-G87 were identified by restriction enzyme digestion and nucleotide sequencing.
对分离到的HEV71阳性分离株进行VP1编码区基因扩增，核苷酸序列测定和同源进化分析。
And identified HEV71 isolates were performed by gene amplification of VP1 coding region, nucleotide sequencing and homology analysis of evolution.
部分扩增产物t A克隆后测定核苷酸序列。
Partial amplification products were sequenced after T-A cloning.
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