标记基因,原本是基因工程的专属名词,但是它已经成为一种基本的实验工具,广泛应用于分子生物学、细胞生物学、发育生物学等方面的研究。 标记基因是一种已知功能或已知序列的基因,能够起着特异性标记的作用。在基因工程意义上来说,它是重组DNA载体的重要标记,通常用来检验转化成功与否;在基因定位意义上来说,它是对目的基因进行标志的工具,通常用来检测目的基因在细胞中的定位。
答:标记基因(marker gene)是一种能够起特异性标记作用的基因。通常用来检验重组DNA载体转化成功与否,或者检测目的基因在植物细胞或组织中的定位。
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释义:多核细胞启动子,多核细胞启动基因(基因标记符号) polykaryocytosis promotor 标记基因 marker gene ; labelledgene ; selectable marker genes 释义:个体基因型标记,个体遗传型标记 idiotypic marker ..
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...nathan等[18]构建的双右边界载体pMarkfree5.0是将bar基因放在双右边界T-DNA序列中,克隆的β-葡萄糖醛酸酶和nptII基因(标记基因)在左边界T-DNA上,得到的转化烟草植株后代中有55.6%的植株抗卡那霉素,利用双右边界载体pMarkfree5.0共转化nptII基因和bar基因的...
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...发机体发生宫颈癌等疾病,以重叠PCR法人工合成HPV16 E6、E7致癌基因的融合基因,以之为目的基因构建了无选择标记基因(Marker-Free)双元载体,期望转..
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First, the safety of marker genes must be considered. Second, a different selectable marker gene must be used when additional foreign genes are introduced into a transgenic plants, but the number of available marker genes is limited.
一方面,必须考虑标记基因的安全性;另一方面,重复多次转化时,当需要转入新的外源基因时必须使用另一种标记基因,而目前可供选择的标记基因种类非常有限,难以满足这种需要。
参考来源 - 双T·2,447,543篇论文数据,部分数据来源于NoteExpress
PCR分子检测与转化的标记基因和外源目的基因抗性三者极有规律性。
The results of PCR molecular detection, marker genes and foreign target-gene with insect-resistance were quite regular.
通过砂培试验研究了标记基因对受体菌株的竞争结瘤能力和固氮有效性的影响。
In the nitrogen-free Pot experiment, the nodulating competitive ability of the parental and marked strains and their nitrogen fixing efficiency were tested.
糖类分解代谢酶基因作为安全标记基因,近年来在植物转化中显示了巨大应用潜力。
The genes coding enzymes that catalyze special sugars have showed big practical potency in plant transformation as safe marker genes.
The best way to label this DNA is by designing probes or labels that hybridize with specific sequences that you're interested in.
最好的标记方法是,设计出能与你感兴趣的,特定基因序列杂交的,分子探针或标签
Suppose that there were some sort of genetic marker and, although we didn't have a tattoo that you would just have to look at, -- but you could have genetic counseling-- have your DNA examined and you could tell, if you had the DNA testing, how much time you had left.
假设有某种基因标记,虽然我们没有,让你不得不去看的在身上的标记了,但你可能会去做基因咨询-,检查你的DNA,如果你做了DNA化验的话,你就能知道自己还剩多少时间。
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