运用正交试验设计理论，对高产L-乳酸突变株NAF-032的最佳摇瓶发酵条件进行了研究，确定了最佳的发酵培养基和发酵条件。

The optimal fermentation medium and conditions of mutant strain NAF-032 with a higher and stable yield of L-lactic acid were studied by orthogonal design theory.

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结果显示，有一个突变株不能分化异形胞； 三个突变株可以形成异形胞，但在缺氮的条件下仍不能维持生长；

The result showed that one mutant was unable to differentiate heterocyst, three formed heterocysts but still could not maintain normal growth, four growed slowly with heterocyst differentiation.

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同时，改变发酵液中不同氮源和碳源，对突变株的发酵条件进行了比较研究。

Also the fermentation conditions were studied with different carbon sources and nitrogen sources.

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