利用胶原酶灌注梯密度离心分离肝星状细胞，用有限稀释法建立大鼠肝星状细胞株ig12。
Hepatic stellate cell was isolated with collagenase perfusion and ladder density centrifuge. Hepatic stellate cell line IG12 was cloned with limited diluted method.
采用有限稀释法对阳生孔进行亚克隆，重复3次，得到两株单克隆细胞株：2E8、3C6。
Positive clone were subcloned to get monoclone cell lines by limit dilution, repeat 3 times, then we got two positive monoelone cell lines named:2E8,3C6.
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