以固相化的SARS抗原淘筛抗体库,并用ELISA检测噬菌体抗体结合SARS病毒的特异性。
Antibodies against SARS virus were screened by biopanning with immobilized virus antigen. The binding specificity of phage antibody to SARS virus was detected by ELISA.
方法用免疫组化SABC法检测五种单克隆抗体,分别使用水煮法、微波法、高压法修复抗原,从中选出最佳修复方法。
Methods Check five kinds of antibody with immunohistochemistry SABC method and chose the best antigen retrieval method among poach method, microwave method and high pressure method respectively.
去糖基化的小麦PGIP不能与纯化的天然PGIP产生的抗体发生免疫学反应,可能是由于小麦PGIP中寡糖链为抗原决定部位。
After deglycosylated, wheat PGTP could not interact with antibody produced by purified native PGtP from wheat. It is the glycans of wheat PGIP that probably fonn the epitope.
应用推荐