②人真皮成纤维细胞的培养(Culture of human dermal fibroblast, Culture of HDFib)：剪去表皮及皮下组织，切成＜1 mm3的真皮组织块，贴附于3.5 cm×6.5 cm的培养瓶底，加1...
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②人真皮成纤维细胞的培养(Culture of human dermal fibroblast, Culture of HDFib)：剪去表皮及皮下组织，切成＜1 mm3的真皮组织块，贴附于3.5 cm×6.5 cm的培养瓶底，加1...
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真皮成纤维细胞的培养 Culture of human dermal fibroblast
目的建立不同组织来源成纤维细胞的培养方法并比较其培养差异。
Objective To set up the method of the culture of human fibroblasts from different sources and compare their discrepancy.
结论：胰酶消化、差速贴壁法是一种适合的心室成纤维细胞的培养方法。
Conclusion: Trypsin enzymic digestion and differential attachment was suitable for culture of ventricular fibroblast.
从人胚胎生殖嵴、肠系膜中消化分离的原始生殖细胞，将其接种在人子宫内膜成纤维细胞饲养层上传代培养。
PGC from genital ridge and mesenterium of human embryo was incubated on fibroblast feeder layers for subculture.
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