...乙肝后肝硬化患者)，应用病例一对照研究和聚合酶链反应-序列特异性引物法(polymerase chain reaction-sequence specific primer，pcr-ssp)，检测icam-1基因g241r(g→a)、k469e(a→g)两个位点以及ifn-γ基因+874t..
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方法 采用聚合酶链反应-序列特异性引物法(polymerase chain reaction with sequence-specific primer,PCR-SSP)对成都地区45例ICP孕妇、18个ICP家庭、45名正常妊娠孕妇及18个正常对照家庭进行HLA-DQ...
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序列特异性引物方法 PCR-SSP
方法运用聚合酶链反应-序列特异性引物（PCR-SSP）法，对38例山东汉族人GPP与94例健康对照进行HLA-DQB1等位基因分型。
Methods The distributing frequencies of HLA-DQB1 alleles were detected with polymerase chain reaction-sequence specific primers (PCR-SSP) in 38 GPP patients and 94 healthy subjects from Shandong.
然后分别提取84例拟进行造血干细胞移植病人及家系成员的外周血d NA，采用RSCA和序列特异性引物体外基因扩增(PCR ssp)法平行对照对HLA A基因位点进行分型。
DNA samples of related hematopoietic stem cell transplant donor-recipients were extracted from peripheral blood cells. HLA-A loci were typed both by RSCA and PCR-SSP.
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