应用放射免疫竞争抑制试验测定抗体的配位特异性，并对抗体的可变区序列进行比较。
METHODS The paratope mapping of antibodies was determined by competitive inhibition in radioimmunoassay and the sequences of variable regions among the antibodies were compared.
方法从Z 10株病毒感染的细胞提取总rna，将逆转录pcr扩增的产物纯化后克隆于t载体并进行序列测定，应用dnastar软件分析比较。
Methods the total RNA were prepared from Z10 virus infected cells and the RT PCR product was cloned into t vector, sequenced and analyzed using DNASTAR software.
方法从Z 10病毒感染的细胞提取总rna，将逆转录聚合酶链反应(RT PCR)扩增的产物纯化后克隆于t载体并进行序列测定，应用dnas TAR软件分析比较。
Methods the total RNA were prepared from Z10 virus infected cells and the RT PCR products was cloned into t vector, sequenced and analyzed by using DNASTAR software.
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