方法：将基因内翻译起始序列合成后克隆到上游含有起始密码和无起始密码的报告质粒中，研究表达水平的差异。

Methods:One of the three internal translation initiation region(TIR) was chemically synthesized and cloned into the report plasmid, and the expression of the report gene was studied.

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亚硫酸氢钠测序检测表达有差异的基因调节序列的甲基化状态。

Bisulfite sequencing was used to determine the methylation status of the regulatory sequence in which gene the expression are significantly difference.

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结果显示，VEGF ASODN对U937细胞的VEGF的表达有明显的抑制作用，与错义序列组和空白对照组相比有显著差异（P<0 .0 5 ） ；

The result showed that VEGF ASODN had obviously inhibitive effect on expression of VEGF in U937 cell, as compared with scrambled sequence and negative control (P<0.05).

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