目的：建立慢性心肌缺血大鼠动物实验模型，探讨动物模型构建的关键技术点。
AIM: to establish a rat model of chronic myocardial ischemia and probe into the experimental techniques for constructing the animal models.
方法：结扎麻醉犬冠状动脉左前降支中段，造成实验性急性心肌缺血模型。
Methods:The animal model of acute myocardial ischemia was reproduced in anesthetized dog by ligating the left coronary artery at the middle of anterior descending branch.
目的采用在体犬心肌缺血再灌注动物实验模型，通过去白细胞技术，研究白细胞在缺血再灌注损伤心肌中产生氧自由基的作用。
Objective To study the role of leukocyte in generating oxygen free radical in myocardial ischemia and reperfusion injury by leukocyte-depletion technique.
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