...大肠杆菌表达系统；水牛；多克隆抗体；BALB/c小鼠[gap=1506]Key words: IFN-γ mature protein gene; Escherichia coli expression system;water buffalo;polyclonal antibody;BALB/c mouse ..
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目的构建可用于大肠杆菌表达系统的含抗冻蛋白基因afp的表达载体。
Objective: to obtain the procaryotic expression vector containing the antifreeze protein gene AFP.
结论成功用大肠杆菌表达系统表达创伤弧菌溶细胞素并对其纯化、复性条件进行优化。
These results indicate that the cytolysin gene from V. vulnificus successfully express in the E. coli system.
创伤弧菌中的溶细胞素在大肠杆菌系统中成功表达。
The cytolysin gene from V. vulnificus successfully express in the E. coli system.
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