为研究IBV变异株的基因组及其变异特征，本实验通过基因扩增拼接的方法获得了肾病变型毒株JH06111的全基因组序列。

To study the genomic characteristics of IBV variant isolate, we obtained the full genome sequences of nephropathogenic JH06111 by amplifying, cloning and sequencing of its cDNA.

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最终，研究人员获得 40 倍的基因组的覆盖面和运用 454 GS 的 De Novo 序列拼接软件（Newbler）绘制出了高质量的草图。

In the end, the researchers obtained 40x genome coverage and generated a high-quality draft assembly using the 454 GS De Novo Assembler Software (Newbler).

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