...grobacteriumtumefaciens-mediated transformation), 电击转化 (electroporation),基因枪转化(biolistic transformation),整细胞转化法等。 1.1.2差异表达和mRNA的差异显示 研究植物病原真菌基因表达的羞异可以克隆到特定过程中的特殊基因。
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4)基因枪转化(Biolistics) 5)农杆菌介导转化(Agrobacterium tumefaciens Mediated Transformation)
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The original transformation model for the seaweed Laminaria japonica (Phaeophyceae) employed female gametophytes as micro-particle bombardment targets and parthenogenesis as a route to produce transgenic parthenosporophytes.
海带遗传转化模型是以基因枪转化海带雌配子体,转化后诱导生成孤雌生殖孢子体。
参考来源 - 海带遗传转化模型的优化及目的基因的导入·2,447,543篇论文数据,部分数据来源于NoteExpress
通过基因枪转化法轰击1160块的愈伤组织得到167株再生植株,转化率17.4%。
By particle bombardment method, 167 regenerated plantlets were gained from 1160 calli, and the transformation frequency was 17.4%.
利用基因枪和农杆菌转化法可将大豆铁结合蛋白基因导入旱稻基因组中,并使转基因稻米铁含量增高。
The soybean ferritin gene can be transferred into upland rice using the method of Biolistic and Agrobacterium-mediated transformation, which increased iron concentration in the transgenic plant seeds.
以悬浮细胞作为基因枪轰击的靶材料,转化植株再生频率较初级愈伤组织的为高。
The efficiency of regenerated plant from resistant clones in suspension cells was higher than that in primary callus.
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