该法又称粒子轰击(particle bombardment)，高速粒子喷射技术(High-velocity particle microprojection)或基因枪轰击技术(gene gun bombardment)，是由美国Cornell大学生物化学系John.C.Sanford等于1983年研究成功。
1．1．1基因枪法（Gene gun-mediated transformation） 基因枪法又称微弹轰击法（Particlebombardment，Biolisticmethod或 Microprojectile bombardment），其基本原理是将DNA包被在微...
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It was suggested that these promoters might be related to plant stress resistance. The promoters driving the beta-glucuronidase (GUS) and green fluorescent protein (GFP) fusion protein were transformed to maize coleoptile using particle bombardment.
为了了解上述启动子的启动强度和表达特异性,构建了带有gus和gfp融合基因的植物表达载体,利用基因枪法轰击玉米胚芽鞘进行瞬时表达分析。
参考来源 - 玉米胁迫相关基因的启动子克隆和多基因表达载体的构建·2,447,543篇论文数据，部分数据来源于NoteExpress
利用基因枪法，以菜豆几丁质酶基因转化小麦幼胚愈伤组织。
Using embryogenic calli from young embryos as explants, bean chitinase gene was transferred into spring wheat plants by microprojectile bombardment.
至今，基因枪法以其实用性和有效性应用于水稻工程育种已有十几年。
Biolistics, by virtue of its overwhelming practicality and effectiveness, has been applied in rice engineering breeding for a dozen years.
目前外源基因导入玉米受体细胞的方法很多，最受瞩目的是农杆菌介导法和基因枪法。
Agrobacterium and gene gun have aroused most attention among various genetic transformation methods for exogenous gene into maize.
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