该技术主要包括：目的基因和靶细胞的制备、样品的灌注以及对细胞的显微注射。
The main procedures were: to prepare the target genes and cells, to fill the sample into the glass capillary injector and to proceed microinjection.
选用较低的基因浓度，良好的操作介质CZB，使用质量好的受精卵，会提高显微注射的效率。
Lower concentration of the microinjected DNA, choosing CZB and zygotes with good quality can enhance efficiency of microinjection.
转基因是近年来在生命科学领域广泛应用的新兴技术，目前80%以上的转基因动物模型是利用显微注射法构建完成的。
Gene transfer is a new and widely used technology in bioscience field. At present over 80 percent of transgenic animal models are constructed by means of microinjection.
应用推荐