目的： 分离培养睾丸支持细胞、骨髓基质细胞、胚胎成纤维细胞和胰腺成纤维细胞，优化培养条件，建立饲养细胞系，为干细胞体外共培养体系的建立提空充足的饲养细胞。

Objective: Sepretae Sertoli cell, BMSC, MEF, Pancreatic fibroblast and establish cell lines, constructed different feeder layers for a source as a coculture system for stem cells.

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其病理生理过程复杂，以胰腺内成纤维细胞和细胞外基质(ecm)在胰腺组织内过多沉积为其特征。

Its pathophysiology is complex, the characteristic is too much sedimentation by fibroblasts and Extracellular matrix (ECM) in the pancreas.

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