目的:在活体上探讨缺血再灌后血管内皮细胞损伤及白细胞、血小板与内皮之间粘附的变化。
Aim: Damage of endothelial cells and adhesion of leukocytes and platelets were studied in rat mesentery microvessels after ischemia reperfusion.
方法利用二维凝胶电泳获得新生隐球菌孵育后血管内皮细胞与正常细胞差异表达蛋白质点,并对部分差异蛋白点进行质谱鉴定分析。
Methods The differentially expressed proteins between HUVEC incubated with Cryptococcus and normal HUVEC were obtained by 2-D electrophoresis and identified by MALDI-TOF MS technology.
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