反相层析，在吸附层析中，低极性物质在层析柱上吸附较牢，洗脱时发生拖尾现象和保留时间长的问题。
公布 中文名称：反相层析 英文名称：reverse phase chromatography 定义：流动相的极性大于固定相的层析技术。分子在此系统中的移动速度依其极性排列，极性大者移动快。 应用学科： 生物化学与分子生物学（一级学科）；方法与技术
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Reversed phase chromatography by C-18 could raise purity of reserpine to 41.8%, but C-18 medium was expensive so that it could not be applied in production.
C-18反相层析法可以得到纯度为41.8%的育亨宾,但C-18介质很贵,在生产上应用有困难。
参考来源 - 从催吐萝芙木中提取利血平和育亨宾的工艺研究·2,447,543篇论文数据，部分数据来源于NoteExpress
采用反相层析图谱和毛细管电泳验证活性成分达到一定纯度。
RP-HPLC and capillary electrophoresis showed that the active fraction had reached some purify.
方法利用反相层析的方法进行分离纯化，根据化合物的化学性质与光谱数据鉴定其结构。
Methods to isolate the constituents by reverse phase chromatography, and characterize their structures by the analysis of chemical property and spectral data.
本文认为当固定相为液体或溶液时，反相纸层析与液液萃取一样属于分配机制。
When the stationary phase is a liquid or a solution partition mechanism governs the retention of reversed-phase paper chromatography just as in liquid-liquid extraction.
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