《内毒素结合肽的改良、原核表达及纯化》论文摘要： 目的:对人内毒素结合肽(endotoxin binding peptide,EBP)进行改良,获得突变体mEBP(mutate of endotoxin binding peptide...
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通过基因克隆等方法，选择原核表达系统，并对目的蛋白进行分离纯化，初步获得有活性的人内毒素结合肽ebp。
We isolated and purified EBP effectively, and obtained EBP with biological activity for the first time with gene cloning method and prokaryotic expression system.
结论：筛选到的短肽lttwapa能特异性结合于内毒素休克小鼠肝脏血管内皮细胞，有可能对内毒素休克的治疗具有重要意义。
CONCLUSION: the peptide of LTTWAPA specifically binds to vascular endothelial cells of liver in septic shock mice, which may have important significance for the therapy of septic shock.
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